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<p>Contributors ix</p> <p>Preface xvii</p> <p><b>I. Genomes, Genomics, and Genetic Exchange</b></p> <p>1. Gene Transfer in <i>Mycobacterium tuberculosis</i>: Shuttle Phasmids to Enlightenment 3<br /><i>William R. Jacobs, Jr.</i></p> <p>2. Mycobacterial Pathogenomics and Evolution 27<br /><i>Daria Bottai, Timothy P. Stinear, Philip Supply, and Roland Brosch</i></p> <p>3. BCG Vaccines 49<br /><i>Vanessa Tran, Jun Liu, and Marcel A. Behr</i></p> <p>4. Distributive Conjugal Transfer: New Insights into Horizontal Gene Transfer and Genetic Exchange in Mycobacteria 61<br /><i>Keith M. Derbyshire and Todd A. Gray</i></p> <p>5. Molecular Genetics of Mycobacteriophages 81<br /><i>Graham F. Hatfull</i></p> <p>6. Genetics of Phage Lysis 121<br /><i>Madalena Pimentel</i></p> <p><b>Ii. Gene Expression and Regulation</b></p> <p>7. Sigma Factors: Key Molecules in <i>Mycobacterium tuberculosis </i>Physiology and Virulence 137<br /><i>Riccardo Manganelli</i></p> <p>8. Transcription Factor Binding Site Mapping Using ChIP-Seq 161<br /><i>Suma Jaini, Anna Lyubetskaya, Antonio Gomes, Matthew Peterson, Sang Tae Park, Sahadevan Raman, Gary Schoolnik, and James Galagan</i></p> <p>9. Noncoding RNA in Mycobacteria 183<br /><i>Kristine B. Arnvig, Teresa Cortes, and Douglas B. Young</i></p> <p>10. Two-Component Regulatory Systems of Mycobacteria 209<br /><i>Tanya Parish</i></p> <p>11. Regulated Expression Systems for Mycobacteria and Their Applications 225<br /><i>Dirk Schnappinger and Sabine Ehrt</i></p> <p><b>Iii. The Mycobacterial Proteome</b></p> <p>12. <i>Mycobacterium tuberculosis </i>in the Proteomics Era 241<br /><i>Martin Gengenbacher, Jeppe Mouritsen, Olga T. Schubert, Ruedi Aebersold, and Stefan H. E. Kaufmann</i></p> <p>13. Structural Annotation of the <i>Mycobacterium tuberculosis </i>Proteome 261<br /><i>Nagasuma Chandra, Sankaran Sandhya, and Praveen Anand</i></p> <p>14. Cyclic AMP Signaling in Mycobacteria 281<br /><i>Gwendowlyn S. Knapp and Kathleen A. McDonough</i></p> <p><b>IV. Metabolism</b></p> <p>15. The Physiology and Genetics of Oxidative Stress in Mycobacteria 299<br /><i>Bridgette M. Cumming, Dirk Lamprecht, Ryan M. Wells, Vikram Saini, James H. Mazorodze, and Adrie J. C. Steyn</i></p> <p>16. Metabolomics of Central Carbon Metabolism in <i>Mycobacterium tuberculosis </i>323<br /><i>Anthony D. Baughn and Kyu Y. Rhee</i></p> <p>17. Mycobacterial Lipidomics 341<br /><i>Emilie Layre, Reem Al-Mubarak, John T. Belisle, and D. Branch Moody</i></p> <p>18. Genetics of Mycobacterial Trehalose Metabolism 361<br /><i>Rainer Kalscheuer and Hendrik Koliwer-Brandl</i></p> <p>19. Metallobiology of Tuberculosis 377<br /><i>G. Marcela Rodriguez and Olivier Neyrolles</i></p> <p>20. Energetics of Respiration and Oxidative Phosphorylation in Mycobacteria 389<br /><i>Gregory M. Cook, Kiel Hards, Catherine Vilcheze, Travis Hartman, and Michael Berney</i></p> <p><b>V. Genetics of Drug Resistance</b></p> <p>21. Molecular Basis of Drug Resistance in <i>Mycobacterium tuberculosis </i>413<br /><i>Keira A. Cohen, William R. Bishai, and Alexander S. Pym</i></p> <p>22. Resistance to Isoniazid and Ethionamide in <i>Mycobacterium tuberculosis</i>: Genes, Mutations, and Causalities 431<br /><i>Catherine Vilcheze and William R. Jacobs, Jr.</i></p> <p>23. The Molecular Genetics of Fluoroquinolone Resistance in <i>Mycobacterium tuberculosis </i>455<br /><i>Claudine Mayer and Howard Takiff</i></p> <p>24. Mechanisms of Pyrazinamide Action and Resistance 479<br /><i>Ying Zhang, Wanliang Shi, Wenhong Zhang, and Denis Mitchison</i></p> <p>25. Genetic Strategies for Identifying New Drug Targets 493<br /><i>Andrej Trauner, Christopher M. Sassetti, and Eric J. Rubin</i></p> <p><b>VI. Genetics of Membrane and Cell Wall Biosynthesis</b></p> <p>26. Genetics of Peptidoglycan Biosynthesis 513<br /><i>Martin S. Pavelka, Jr., Sebabrata Mahapatra, and Dean C. Crick</i></p> <p>27. Genetics of Mycobacterial Arabinogalactan and Lipoarabinomannan Assembly 535<br /><i>Monika Jankute, Shipra Grover, Helen L. Birch, and Gurdyal S. Besra</i></p> <p>28. Genetics of Capsular Polysaccharides and Cell Envelope (Glyco)lipids 559<br /><i>Mamadou Daffe, Dean C. Crick, and Mary Jackson</i></p> <p>29. The Molecular Genetics of Mycolic Acid Biosynthesis 611<br /><i>Jakub Pawełczyk and Laurent Kremer</i></p> <p><b>VII. Genetics of Macromolecular Biosynthesis</b></p> <p>30. Nucleotide Metabolism and DNA Replication 635<br /><i>Digby F. Warner, Joanna C. Evans, and Valerie Mizrahi</i></p> <p>31. Double-Strand DNA Break Repair in Mycobacteria 657<br /><i>Michael S. Glickman</i></p> <p>32. The Pup-Proteasome System of Mycobacteria 667<br /><i>Nadine J. Bode and K. Heran Darwin</i></p> <p>33. <i>Mycobacterium tuberculosis </i>Serine/Threonine Protein Kinases 681<br /><i>Sladjana Prisic and Robert N. Husson</i></p> <p><b>VIII. The Mycobacterial Lifestyle, Persistence, and Macrophage Survival</b></p> <p>34. The Spectrum of Drug Susceptibility in Mycobacteria 711<br /><i>Bree B. Aldridge, Iris Keren, and Sarah M. Fortune</i></p> <p>35. The Sculpting of the <i>Mycobacterium tuberculosis </i>Genome by Host Cell–Derived Pressures 727<br /><i>David G. Russell, Wonsik Lee, Shumin Tan, Neelima Sukumar, Maria Podinovskaia, Ruth J. Fahey, and Brian C. VanderVen</i></p> <p>36. Evasion of Innate and Adaptive Immunity by <i>Mycobacterium tuberculosis </i>747<br /><i>Michael F. Goldberg, Neeraj K. Saini, and Steven A. Porcelli</i></p> <p>37. Mycobacterial Biofilms 773<br /><i>Jacobs P. Richards and Anil K. Ojha</i></p> <p>Index 785</p>

<p><b>Molecular Genetics of Mycobacteria</b> <p>Second Edition <p><i>A comprehensive collection of perspectives by experts in mycobacterial molecular biology</i> <p> Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today. <p> Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge. <p> Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition, <ul> <li> Discusses key areas of current research in mycobacterial genetics</li> <li>Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis</li> <li>Presents genetic approaches for manipulating M. tuberculosis</li> </ul> <p> This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria.

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